基本情况
许永男，男，吉林和龙人，中共党员。现任五邑大学药学与食品工程学院专任教师。
联系方式
地址：广东省江门市蓬江区东成村22号 五邑大学药学与食品工程学院
邮编：529020
邮箱：ynxu0613@163.com;
ynxu0613@wyu.edu.cn
工作经历
2021年12月至今，五邑大学，药学与食品工程学院，硕士生导师
2014年09月至2021年11月，延边大学，农学院生物技术专业，硕士生导师
2014年01月至2014年08月，韩国忠北大学，农业生命环境科学院，研究教授
2012年03月至2013年12 月，韩国忠北大学，农业生命环境科学院，博士后
教育及学习经历
2008年03月至2012年02月，韩国忠北大学，农业生命环境科学院，博士
2005年09月至2008年02月，韩国忠北大学，农业生命环境科学院，硕士
2000年09月至2004年07月，延边大学农学院， 学士
主要研究方向
1、哺乳动物卵母细胞成熟及胚胎发育机制研究
2、转基因动物生产及应用研究
发表学术论文
First author or Corresponding author
1. He-Wei Ji, Chao-Rui Wang, Xiu-Wen Yuan, Jing Wang, Lin Wang, Qi-Long Cao, Ying-Hua Li, Yong-Nan Xu*, Nam-Hyung Kim. Mangiferin improves early porcine embryonic development by reducing oxidative stress. Reprod Domest Anim. 2024 Apr;59(4):e14565.
2. Jing Wang, Ying-Hua Li, Rong-Ping Liu, Xin-Qin Wang, Mao-Bi Zhu, Xiang-Shun Cui Zhen Dai, Nam-Hyung Kim and Yong-Nan Xu* . Supplementation with Eupatilin during In Vitro Maturation Improves Porcine Oocyte Developmental Competence by Regulating Oxidative Stress and Endoplasmic Reticulum Stress. Animals (Basel). 2024 Jan 30;14(3):449.
3. Xuerui Yao, Panpan Guo, Ying-Hua Li, Hao Guo, Zhelong Jin, Wen Lui, Jianbin Yuan, Qingshan Gao, Lin Wang, Yunxiao Li, Jiabin Shi, Xiwei Zhang, Qilong Cao, Yong-Nan Xu*, Nam-Hyung Kim*. Apigenin delays postovulatory oocyte aging by reducing oxidative stress through SIRT1 upregulation. Theriogenology. 2024 Jan 7:218:89-98.
4. Jing Wang , Xin-Qin Wang , Rong-Ping Liu , Ying-Hua Li , Xue-Rui Yao , Nam-Hyung Kim*, Yong-Nan Xu*. Melatonin Supplementation during In Vitro Maturation of Porcine Oocytes Alleviates Oxidative Stress and Endoplasmic Reticulum Stress Induced by Imidacloprid Exposure. Animals (Basel). 2023 Aug 11;13(16):2596.
5. Rong-Ping Liu, Sheng-Yan He, Jing Wang, Xin-Qin Wang, Zhe-Long Jin, Hao Guo, Chao-Rui Wang, Yong-Nan Xu*, Nam-Hyung Kim*. BDE-47 Induces Mitochondrial Dysfunction and Endoplasmic Reticulum Stress to Inhibit Early Porcine Embryonic Development. Animals (Basel). 2023 Jul 13;13(14):2291.
6. Yong-Nan Xu, Guo-Bo Han, Ying-Hua Li, Chun-Hao Piao, Guan-Hao Li, Nam-Hyung Kim. Protective effect of onion peel extract on ageing mouse oocytes. Zygote. 2023 Oct;31(5):451-456.
7. Jing Wang, Rong-Ping Liu, Xin-Qin Wang, Ying-Hua Li, Nam-Hyung Kim*, Yong-Nan Xu*. Dihydromyricetin supplementation during in vitro culture improves porcine oocyte developmental competence by regulating oxidative stress. J Reprod Dev. 2023 Feb 8;69(1): 10–17.
8. Xin-Qin Wang, Rong-Ping Liu, Jing Wang, Dan Luo, Ying-Hua Li, Hao Jiang, Yong-Nan Xu*, Nam-Hyung Kim*.Wedelolactone facilitates the early development of parthenogenetically activated porcine embryos by reducing oxidative stress and inhibiting autophagy. Peer J. 2022 Jul 25:10:e13766.
9. Rong-Ping Liu, Xin-Qin Wang, Jing Wang, Luo Dan, Ying-Hua Li, Hao Jiang, Yong-Nan Xu*, Nam-Hyung Kim*. Oroxin A reduces oxidative stress, apoptosis, and autophagy and improves the developmental competence of porcine embryos in vitro. Reprod Domest Anim. 2022 Oct;57(10):1255-1266.
10. Wen-Jie Jiang, Wen Liu, Ying-Hua Li, Hao Jiang, Yong-Nan Xu*, Nam-Hyung Kim*. Citrinin impairs pig oocyte maturation by inducing oxidative stress and apoptosis. Toxicon. 2022 Jan 15;205:84-90.
11. 韩国波，李英花，叶孟傲然，许永男*。 细胞周期检查点激酶1 的表达对猪卵母细胞体外成熟的影响。畜牧与兽医。 2020年 第52卷 第11期。
12. Xuerui Yao, Hao Jiang, Qingshan Gao, Ying-Hua Li, Yong Nan Xu*, Nam-Hyung Kim*. Melatonin alleviates defects induced by zearalenone during porcine embryo development. Theriogenology.2020 Jul 15;151:66-73.
13. Wen-Jie Jiang, Lin-Lin Hu, Yan-Ping Ren, Xiang Lu, Xiao-Qiong Luo, Ying-Hua Li*, Yong-Nan Xu*. Podophyllotoxin affects porcine oocyte maturation by inducing oxidative stress-mediated early apoptosis. Toxicon. 2020 Mar;176:15-20
14. Wenjie Jiang, Yinghua Li, Yuhan Zhao, Qingshan Gao, Qingguo Jin, Changguo Yan**, Yongnan Xu*. L-carnitine supplementation during in vitro culture regulates oxidative stress in embryos from bovine aged oocytes. Theriogenology. 2020 Feb; Vol143; 64-73
15. Xuerui Yao, Hao Jiang, Ying‐Hua Li, Qingshan Gao, Yong Nan Xu*, Nam‐Hyung Kim*. Kaempferol alleviates the reduction of developmental competence during aging of porcine oocytes. Anim Sci J. 2019 Nov; 90(11): 1417-1425
16. Wenjie Jiang, Xuerui Yao, Yuhan Zhao, Qingshan Gao, Qingguo Jin, Yinghua Li, Changguo Yan, Yongnan Xu*. L-carnitine prevents bovine oocyte aging and promotes subsequent embryonic development. J Reprod Dev. 2019 Dec 18;65(6):499-506.
17. 王华清，高青山，姜文杰，赵予晗，金庆国，李英花，许永男*。Psammaplin A对牛老化卵母细胞体外发育的影响。中国畜牧兽医。2019,46(2):497-503。
18. 许永男。动物生物技术人才培养的实践与思考。2018年第37期。
19. Yao X, Jiang H, Liang S, Shen X, Gao Q, Xu YN*, Kim NH*. Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress. J Reprod Dev. 2018 Dec 14;64(6):489-494.
20. 姜文杰，李英花，姚雪瑞，赵予晗，高青山，朴春浩，孙福亮，许永男*。冷,热应激对猪孤雌胚胎体外发育的影响。中国畜牧兽医。2018, 45 (9): 2507-2515。
21. Yu Wu, Na Zhang, Ying-Hua Li, Liang Zhao, Mo Yang, Yimei Jin, Yong-Nan Xu* and Hongyan Guo*. Citrinin exposure affects oocyte maturation and embryo development by inducing oxidative stress-mediated apoptosis. Oncotarget. 2017 May 23; 8(21): 34525-34533.
22. Heo YT, Quan X, Xu YN, Baek S, Choi H, Kim NH, Kim J. CRISPR/Cas9 nuclease-mediated gene knock-in in bovine-induced pluripotent cells. Stem Cells Dev. 2015 Feb 1;24(3):393-402.
23. Xu YN, Li YH, Cui XS, Kim NH. Hyperglycemia Influences Apoptosis and Autophagy in Porcine Parthenotes Developing In Vitro. Reprod Dev Biol. 2013 37(2): 65-73.
24. Xu YN, Uhm SJ, Koo BC, Kwon MS, Roh JY, Yang JS, Choi HY, Heo YT, Cui XS, Yoon JH, Kim T, Kim NH. Production of Transgenic Korean Native Cattle Expressing Enhanced Green Fluorescent Protein Using a FIV-based Lentiviral Vector Injected into MII Oocytes. J Genet Genomics. 2013 Jan 20; 40(1): 37-43.
25. Xu YN, Shen XH, Lee SE, Kim DJ, Heo YT, Cui XS, Kim NH. Autophagy influences maternal mRNA degradation and apoptosis in porcine parthenotes developing in vitro. J Reprod Dev. 2012; 58(5): 576-584.
26. Cui XS, Xu YN (共同一作), Shen XH, Zhang LQ, Zhang JB, Kim NH. Trichostatin A modulates apoptotic-related gene expression and improves embryo viability in cloned bovine embryos. Cell Reprogram. 2011 Apr;13(2):179-89.
27. Xu YN, Cui XS, Sun SC, Lee SE, Kim NH. Cross species fertilization and development investigated by cat sperm injection into mouse oocytes. J Exp Zool A Ecol Genet Physiol. 2011 Jul 1;315(6):349-57.
28. Xu YN, Cui XS, Tae JC, Jin YX, Kim NH. DNA synthesis and epigenetic modification during mouse oocyte fertilization by human or hamster sperm injection. J Assist Reprod Genet. 2011 Apr; 28(4): 325-333.
29. Xu YN, Cui XS, Sun SC, Lee SE, Li YH, Kwon JS, Lee SH, Hwang KC, Kim NH. Mitochondrial dysfunction influences apoptosis and autophagy in porcine parthenotes developing in vitro. J Reprod Dev. 2011 Feb;57(1):143-50.
Co-author
30. Chao-Rui Wang, Xiu-Wen Yuan, He-Wei Ji, Yong-Nan Xu, Ying-Hua Li*, Nam-Hyung Kim*. Chrysoeriol Improves the Early Development Potential of Porcine Oocytes by Maintaining Lipid Homeostasis and Improving Mitochondrial Function. Antioxidants (Basel). 2024 Jan 19;13(1):122
31. Zhe-Long Jin, KangHe Xu, Jonghun Kim, Hao Guo, Xuerui Yao, Yong-Nan Xu, Ying-Hua Li, DongHee Ryu, Kee-Pyo Kim, Kwonho Hong, Yong-June Kim, Lin Wang, Qilong Cao, Kyun-Hwan Kim, Nam-Hyung Kim*, Dong Wook Han*. 3D hepatic organoid production from human pluripotent stem cells. Differentiation. 2023 Dec 12:135:100742.
32. Xuerui Yao, Ji Hyun Kang, Kee-Pyo Kim, Hyogeun Shin, Zhe-Long Jin, Hao Guo, Yong-Nan Xu, Ying-Hua Li, Sai Hali, Jeongwoo Kwon, Hyeonwoo La, Chanhyeok Park, Yong-June Kim, Lin Wang, Kwonho Hong, Qilong Cao, Il-Joo Cho, Dong Wook Han*, Nam-Hyung Kim*. Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells. Stem Cells Int. 2023 Sep 29:2023:3320211.
33. Sheng-Yan He, Rong-Ping Liu, Chao-Rui Wang, Xin-Qin Wang, Jing Wang, Yong-Nan Xu, Nam-Hyung Kim, Dong-Wook Han*, Ying-Hua Li*. Improving the developmental competences of porcine parthenogenetic embryos by Notoginsenoside R1-induced enhancement of mitochondrial activity and alleviation of proapoptotic events. Reprod Domest Anim. 2023 Nov;58(11), 1583-1594.
34. Dong Wook Han, KangHe Xu, Zhe-Long Jin, Yong-Nan Xu, Ying-Hua Li, Lin Wang, Qilong Cao, Kee-Pyo Kim, DongHee Ryu, Kwonho Hong, Nam-Hyung Kim*. Customized liver organoids as an advanced in vitro modeling and drug discovery platform for non-alcoholic fatty liver diseases.Int J Biol Sci. 2023;19(11):3595-3613.
35. Rong-Ping Liu, Jing Wang, Xin-Qin Wang, Chao-Rui Wang, Sheng-Yan He, Yong-Nan Xu, Ying-Hua Li, Nam-Hyung Kim. Xanthoangelol promotes early embryonic development of porcine embryos by relieving endoplasmic reticulum stress and enhancing mitochondrial function. Reprod Biomed Online. 2023 Aug;47(2):103211.
36. Chao-Rui Wang , He-Wei Ji, Sheng-Yan He, Rong-Ping Liu, Xin-Qin Wang, Jing Wang, Chu-Man Huang, Yong-Nan Xu, Ying-Hua Li* and Nam-Hyung Kim*. Chrysoeriol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress and Autophagy. Vet Sci. 2023 Feb 10;10(2):143.
37. Panpan Guo , Xuerui Yao , Xin Jin , Yongnan Xu , Junfang Zhang , Qiang Li , Changguo Yan , Xiangzi Li , Nam-Hyung Kim. Interference with DGAT Gene Inhibited TAG Accumulation and Lipid Droplet Synthesis in Bovine Preadipocytes. Animals (Basel). 2023 Jul 6;13(13):2223.
38. Wenjun Zhou, Ying-Jie Niu, Zheng-Wen Nie, Ju-Yeon Kim, Yong-Nan Xu, Chang-Guo Yan, Xiang-Shun Cui. Nuclear accumulation of pyruvate dehydrogenase alpha 1 promotes histone acetylation and is essential for zygotic genome activation in porcine embryos. Biochim Biophys Acta Mol Cell Res. 2020 Apr;1867(4):118648.
39. Ying-Jie Niu , Wenjun Zhou , Zheng-Wen Nie , Dongjie Zhou , Yong-Nan Xu, Sun A Ock, Chang-Guo Yan, Xiang-Shun Cui. Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs. Aging. 2020 Jan 20;12(2):1256-1271
40. Zhao Y, Xu Y, Li Y, Jin Q, Sun J, E Z, Gao Q. Supplementation of kaempferol to in vitro maturation medium regulates oxidative stress and enhances subsequent embryonic development in vitro. Zygote. 2020 Feb;28(1):59-64.
41. 何道宏，韩国波，孙敬宇，鄂志强，崔宇新，许永男，高青山。芝麻素对小鼠早期胚胎体外发育的影响。中国畜牧兽医。2019,46（12）：3666-3673
42. 赵予晗，姚雪瑞，姜文杰，王华清，许永男，高青山。添加Ge-132对牛体外胚胎发育潜能的影响。中国畜牧兽医。2018,45(9):2537-2543。
43. 姚雪瑞，许永男，金庆国，李英花，高青山。不同培养体系对延边黄牛卵母细胞体外受精胚胎发育率的影响。2017(08上):111-113。
44. 金贞爱，姚雪瑞，高青山，许永男，李英花。延边黄牛胚胎移植技术研究。2016年12月第44卷第6期。
45. Ying-Hua Li, Yong-Nan Xu, Zi-Li Lin, JeongWoo Kwon, Xiang-Shun Cui and Nam-Hyung Kim. Inhibition of the Arp2/3 complex impairs early embryo development of porcine parthernotes. Animal cell and systems. 2016 Vol. 20. NO 5. 253-259.
46. Lee SR, Xu YN, Jo YJ, Namgoong S, Kim NH. The Rho-GTPase effector ROCK regulates meiotic maturation of the bovine oocyte via myosin light chain phosphorylation and cofilin phosphorylation. Mol Reprod Dev. 2015 Jul 15.
47. Lin Z, Xu YN, Namgoong S, Kim NH. JMY function as actin nucleation-promoting factor and mediator for p53-mediated DNA damage in porcine oocytes. PLoS One. 2014 Oct 3;9 (10):e109385.
48. Lin ZL, Li YH, Xu YN, Wang QL, Namgoong S, Kim NH. Effects of Growth Differentiation Factor 9 and Bone Morphogenetic Protein 15 on thein vitro Maturation of Porcine Oocytes. Reprod Domest Anim. 2014 Apr;49(2):219-27.
49. Heo YT,Lim JK, Xu YN, Jang WI, Jeon SH, Kim NH. Development of a method of vitrification, thawing, and transfer of mammalian blastocysts using a single closed cryo-straw. Cryo Letters. 2014 Mar-Apr; 35(2):108-13.
50. Li YH, Kang H, Xu YN, Heo YT, Cui XS, Kim NH, Oh JS. Greatwall kinase is required for meiotic maturation in porcine oocytes. Biol Reprod. (2013) 89(3):53, 1–7.
51. Sun SC, Wang QL, Gao WW, Xu YN, Liu HL, Cui XS, Kim NH. Actin nucleator Arp2/3 complex is essential for mouse preimplantation embryo development. Reproduction, Fertility and Development. 2013;25(4):617-623. (IF=2.109)
52. Lee SH, Xu YN, Heo YT, Cui XS, Kim NH. Effects of Trichostatin A and 5-aza-2’deoxycytidine on Nuclear Reprogramming in Pig Cloned Embryos. Reprod Dev Biol. 2013 37(4):269-279.
53. Jeon SH, Jung KS, Choi JW, Heo YT, Xu YN, Kim NH. Optimization of In Vivo Embryo Production and Pregnancy following Embryo Transfer in Hanwoo Cattle. J. Emb. Trans. 2013 Vol. 28, No.4, pp. 307-314.
54. Choi YH, Lee SH, Xu YN, Lee SE, Kim NH. Caffeine Treatment during Oocyte Aging Improves the Developmental Rate and Quality in Bovine Embryos Developing In Vitro. Reprod Dev Biol. 2013 37(4):281-287.
55. Sun SC, Gao WW, Xu YN, Jin YX, Wang QL, Yin XJ, Cui XS, Kim NH. Degradation of actin nucleators affects cortical polarity of aged mouse oocytes. Fertil Steril. 2012 Apr;97(4):984-990. (IF=3.122)
56. Jin YX, Cui XS, Yu XF, Lee SH, Wang QL, Gao WW, Xu YN, Sun SC, Kong IK, Kim NH. Cat fertilization by mouse sperm injection. Zygote. 2012 Nov;20(4):371-8. (IF=1.241)
57. Lee SE, Hwang KC, Sun SC, Xu YN, Kim NH. Modulation of autophagy influences development and apoptosis in mouse embryos developing in vitro. Mol Reprod Dev. 2011 Jul 78(7):498-509 (IF=2.395)
58. Sun SC, Zhang DX, Lee SE, Xu YN, Kim NH. Ndc80 regulates meiotic spindle organization, chromosome alignment, and the spindle assembly checkpoint in mouse oocytes. Microsc Microanal. 2011 Jun; 17(3):431-439. (IF=2.179)
59. Sun SC, Xu YN, Li YH, Lee SE, Jin YX, Cui XS, Kim NH. WAVE2 regulates meiotic spindle stability, peripheral positioning and polar body emission in mouse oocytes. Cell Cycle. 2011 Jun 1; 10(11):1853-1860. (IF=4.999)
60. Sun SC, Wang ZB, Lee SE, Xu YN, Cui XS, Kim NH. Arp2/3 complex regulates asymmetric division and cytokinesis in mouse oocytes. PLoS ONE. 2011 Apr 8; 6(4): e18392 (IF=4.411)
61. Sun SC, Lee SE, Xu YN, Kim NH. Perturbation of Spc25 expression affects meiotic spindle organization, chromosome alignment, and the spindle assembly checkpoint in mouse oocytes. Cell Cycle. 2010 Nov 15; 9(22):4552-4559. (IF=4.999)
62. Zhang DX, Park WJ, Sun SC, Xu YN, Li YH, Cui XS, Kim NH. Regulation of maternal gene expression by MEK/MAPK and MPF signaling in porcine oocyte during in vitro meiotic maturation. J Reprod Dev. 2011 Feb; 57(1):49-56. (IF=1.637)
主持的项目
1. 2022年度教育部《春晖计划》合作科研项目(广东省教育厅)：Eupatilin对猪卵母细胞成熟和胚胎发育的影响；立项编号：HZKY20220108；项目编号：202202131；1万；2023.05-2025.05.21；主持
2. 中国科学园广州生物医药与健康研究院（开放课题）；Eupatilin对猪卵母细胞成熟和胚胎发育的影响；项目编号：KLRB202204；2万；2023.01-2024.12；主持
3. 五邑大学高层次人才启动基金项目；瘦素（leptin）对猪卵母细胞成熟、胚胎发育及胚胎冷冻的作用机制研究；项目编号：2021AL023；100万；2022.01-2026.12；主持
4. 国家外国专家局项目；血管紧张素转换酶2（ACE2）基因编辑猪生产及应用；项目编号：G2021030028L；40万；2021.01-2022.12；主持
5. 吉林省教育厅“十三五”科学技术项目;海带多糖对牛卵母细胞和胚胎抗氧化机理的研究；项目编号：JJKH20191132KJ；2.5 万；2019.01-2020.12；主持.
6. 吉林省教育厅“十三五”科学技术项目，提高延边黄牛克隆胚胎发育率的研究，项目编号：［2016］第256号；3 万；2016.01-2017.12；主持
申请及授权专利
1. 姚雪瑞、金南衡、李英花、许永男、郭盼盼、金哲龙。芹菜素在制备抗衰老药物中的应用。专利号 2023117764543；2023.11.17申请
2. 金南衡、韩东旭、金哲龙、姚学瑞、郭浩、许永男、李英花。具有与实际肝脏组织相似的结构和功能的微型肝脏类器官的生产方法。专利号 2023109804712；2023.8.7申请
3. 李英花、何盛艳、刘容萍、冀荷玮、许永男、金南衡。三七皂苷R1在制备促进体外胚胎发育产品中的应用。专利号 2023108613262；2023.7.13申请
4. 李英花、何盛艳、刘容萍、金南衡、许永男。 黄当归醇在制备用于促进胚胎发育产品中的应用。专利号 2023104235880；2023.4.18申请
5. 金南衡、冀荷玮、刘容萍、何盛艳、李英花、许永男。4-苯基丁酸在制备提高体外胚胎质量产品中的应用。专利号 2023108612876；2023.7.13申请
6. 李英花、王薪钦、金南衡、许永男、王晶、刘容萍。黄芪甲苷在制备促进囊胚形成和/或提高囊胚质量产品中的应用。专利号 202310618037X；2023.5.29申请
7. 金南衡、王朝睿、许永男、李英花。金圣草素在制备促进胚胎发育产品中的应用。专利号 2022117342797；2022.12.30申请
8. 许永男、孟子龙、金南衡、李英花、韩国波。洋葱皮提取物在延缓卵母细胞老化中的应用。专利号 2023101853157 2023.2.28申请
9. 金南衡、王晶、王薪钦、刘容萍、许永男、李英花。二氢杨梅素或其衍生物在制备提高体外胚胎质量产品中的应用。专利号 2022108837413；2022.7.26申请
10. 金南衡、刘容萍、王晶、王薪钦、许永男、李英花。木蝴蝶苷A或其类似物在制备提高体外胚胎质量产品中的应用。专利号 2022108824377；2022.7.26申请
11. 金南衡、王薪钦、刘容萍、王晶、李英花、许永男。蟛蜞菊内酯在制备促进胚胎发育产品中的应用。专利号 2022107528821；2022.6.29申请；2023.10.17授权
获奖及荣誉
1, 2018年第五届“生泰尔杯”大学生动物医学专业技能大赛一等奖，被评为优秀指导教师
2, 2012年第9届亚洲生殖生物技术大会最佳海报奖
3, 获韩国BK21教育研究中心奖学金（2006-2011）
